NADH diaphorase polymorphism in goat erythrocytes
نویسندگان
چکیده
منابع مشابه
NAD(P) glycohydrolase deficiency in human erythrocytes and alteration of cytosol NADH-methemoglobin diaphorase by membrane NAD-glycohydrolase activity.
Erythrocytic NADH methemoglobin diaphorase acquires NADH-dichlorophenolindophenol diaphorase activity when enzyme-associated NAD is removed. This transformation is reversible and can be mediated by membrane NAD glycohydrolase (EC 3.2.2.5) in hemolysates as well as in intact cells exposed to hydrogen peroxide. It is abolished either in NADH methemoglobin diaphorase deficiency or in NAD(P) glycoh...
متن کاملPurification and properties of diphosphopyridine nucleotide diaphorase of human erythrocytes.
Diaphorase Assay-Six-hundredths pmole of 2,6-dichlorophenolindophenol, 50 pmoles of Tris-HCl, pH 7.55, 1 pmole of EDTA, enzyme, and 0.44 pmole of DPNH in a volume of 3 ml were placed in a Beckman spectrophotometer equipped with a water jacket through which water at 25” was circulated. A control cuvette without enzyme was included, and absorbancy at 600 rnp was determined for 10 minutes. Under t...
متن کاملAlteration of NADH-diaphorase and cytochrome b5 reductase activities of erythrocytes, platelets, and leucocytes in hereditary methaemoglobinaemia with and without mental retardation.
NADH-diaphorase and cytochrome b5 reductase activities of platelets and leucocytes, as well as erythrocytes, were found to be deficient in a patient with hereditary methaemoglobinaemia associated with moderate mental retardation and non-progressive neurological disturbance, in which hyperactive reflexes and involuntary movements were notable. In another methaemoglobinaemic patient with no menta...
متن کاملA period of appearance of NADH-diaphorase activity in mitotic cycle of Ehrlich's ascites tumor cells.
NADH-diaphorase, one of flavoenzymes, is known to lose scarcely its activity in riboflavin deficiency. In that case, as reported previously (1), the enzyme still has its activity even in the intestinal mucosa, a very actively regenerating tissue. We therefore assumed that this enzyme increased its activity directly before mitosis. In order to confirm this assumption we have studied the activity...
متن کاملElectron microscopic study of the polymyxin treated goat erythrocytes.
Polymyxin B produced dose dependent changes in the surface topography of the goat erythrocyte cells. Transformation from the normal biconcave discs through crenated structures to the final rounded or spherical shape was recorded by scanning electron microscopy. A maximum of three to four crenations per cell was recorded corresponding to a polymyxin dose of 15.62 micrograms/ml. Transmission elec...
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ژورنال
عنوان ژورنال: Animal Genetics
سال: 2009
ISSN: 0268-9146,1365-2052
DOI: 10.1111/j.1365-2052.1987.tb00769.x